human ngf Search Results


human b ngf antibody  (R&D Systems)
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R&D Systems human b ngf antibody
Human B Ngf Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human β ngf  (R&D Systems)
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R&D Systems human β ngf
Human β Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ngf  (R&D Systems)
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R&D Systems ngf
Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human β ngf  (R&D Systems)
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R&D Systems recombinant human β ngf
Recombinant Human β Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mab256  (R&D Systems)
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human ngf  (R&D Systems)
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R&D Systems human ngf
Human Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat pab anti human β ngf  (R&D Systems)
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R&D Systems goat pab anti human β ngf
Goat Pab Anti Human β Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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conventional duoset kits  (R&D Systems)
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ngf β  (R&D Systems)
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R&D Systems ngf β
Ngf β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ngf elisa kit  (Biosensis ltd)
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Biosensis ltd human ngf elisa kit
Three-dimensional (3D) SLCs exhibit enhanced therapeutic potential in vitro. The relative secretion of <t>NGF</t> (A) and GDNF (B) in the supernatant was analyzed by an <t>enzyme-linked</t> <t>immunosorbent</t> <t>assay</t> in the ADSC, conventional SLC, and 3D SLC groups (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. (C) Representative images of SH-SY5Y cells after direct co-culture with ADSCs, conventional SLCs, and 3D SLCs. The neurites were visualized by immunofluorescent labeling of βIII tubulin (green). (D) Statistical analyses of the number of neurites per cell (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. (E) Statistical analyses of the relative length of each neurite (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. Scale bar in C, 50 μm. SLCs: Schwann-like cells; NGF: nerve growth factor; GDNF: glial cell-derived neurotrophic factor; ADSC: Adipose-derived stem cell; DAPI, 4,’6-diamidino-2-phenylindole, dihydrochloride.
Human Ngf Elisa Kit, supplied by Biosensis ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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enzyme immunoassay sandwich kit  (Elabscience Biotechnology)
93
Elabscience Biotechnology enzyme immunoassay sandwich kit
Three-dimensional (3D) SLCs exhibit enhanced therapeutic potential in vitro. The relative secretion of <t>NGF</t> (A) and GDNF (B) in the supernatant was analyzed by an <t>enzyme-linked</t> <t>immunosorbent</t> <t>assay</t> in the ADSC, conventional SLC, and 3D SLC groups (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. (C) Representative images of SH-SY5Y cells after direct co-culture with ADSCs, conventional SLCs, and 3D SLCs. The neurites were visualized by immunofluorescent labeling of βIII tubulin (green). (D) Statistical analyses of the number of neurites per cell (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. (E) Statistical analyses of the relative length of each neurite (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. Scale bar in C, 50 μm. SLCs: Schwann-like cells; NGF: nerve growth factor; GDNF: glial cell-derived neurotrophic factor; ADSC: Adipose-derived stem cell; DAPI, 4,’6-diamidino-2-phenylindole, dihydrochloride.
Enzyme Immunoassay Sandwich Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human recombinant ngf  (R&D Systems)
94
R&D Systems human recombinant ngf
Three-dimensional (3D) SLCs exhibit enhanced therapeutic potential in vitro. The relative secretion of <t>NGF</t> (A) and GDNF (B) in the supernatant was analyzed by an <t>enzyme-linked</t> <t>immunosorbent</t> <t>assay</t> in the ADSC, conventional SLC, and 3D SLC groups (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. (C) Representative images of SH-SY5Y cells after direct co-culture with ADSCs, conventional SLCs, and 3D SLCs. The neurites were visualized by immunofluorescent labeling of βIII tubulin (green). (D) Statistical analyses of the number of neurites per cell (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. (E) Statistical analyses of the relative length of each neurite (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. Scale bar in C, 50 μm. SLCs: Schwann-like cells; NGF: nerve growth factor; GDNF: glial cell-derived neurotrophic factor; ADSC: Adipose-derived stem cell; DAPI, 4,’6-diamidino-2-phenylindole, dihydrochloride.
Human Recombinant Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Three-dimensional (3D) SLCs exhibit enhanced therapeutic potential in vitro. The relative secretion of NGF (A) and GDNF (B) in the supernatant was analyzed by an enzyme-linked immunosorbent assay in the ADSC, conventional SLC, and 3D SLC groups (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. (C) Representative images of SH-SY5Y cells after direct co-culture with ADSCs, conventional SLCs, and 3D SLCs. The neurites were visualized by immunofluorescent labeling of βIII tubulin (green). (D) Statistical analyses of the number of neurites per cell (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. (E) Statistical analyses of the relative length of each neurite (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. Scale bar in C, 50 μm. SLCs: Schwann-like cells; NGF: nerve growth factor; GDNF: glial cell-derived neurotrophic factor; ADSC: Adipose-derived stem cell; DAPI, 4,’6-diamidino-2-phenylindole, dihydrochloride.

Journal: Cell Transplantation

Article Title: Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol

doi: 10.1177/09636897221093312

Figure Lengend Snippet: Three-dimensional (3D) SLCs exhibit enhanced therapeutic potential in vitro. The relative secretion of NGF (A) and GDNF (B) in the supernatant was analyzed by an enzyme-linked immunosorbent assay in the ADSC, conventional SLC, and 3D SLC groups (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. (C) Representative images of SH-SY5Y cells after direct co-culture with ADSCs, conventional SLCs, and 3D SLCs. The neurites were visualized by immunofluorescent labeling of βIII tubulin (green). (D) Statistical analyses of the number of neurites per cell (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. (E) Statistical analyses of the relative length of each neurite (n = 4). Data are expressed as means ± SD. * P < 0.05; ** P < 0.01, one-way ANOVA with Tukey’s post-test. Scale bar in C, 50 μm. SLCs: Schwann-like cells; NGF: nerve growth factor; GDNF: glial cell-derived neurotrophic factor; ADSC: Adipose-derived stem cell; DAPI, 4,’6-diamidino-2-phenylindole, dihydrochloride.

Article Snippet: Next, Human NGF ELISA kit (BEK-2212-1P; Biosensis, Thebarton, Australia) and Human Glial Cell-Derived Neurotrophic Factor (GDNF) ELISA kit (BEK-2222-1P; Biosensis) were used to assess NGF and GDNF secretion in supernatants according to the manufacturer’s instructions.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Labeling, Derivative Assay